ORCID site


A fluorogenic chemically induced dimerization technology for controlling, imaging and sensing protein proximity
S. Bottone, Z.V. Cakil, O. Joliot, G. Boncompain, F. Perez & A. Gautier


Fluorescence-activating and absorption-shifting tags for advanced imaging and biosensing
Accounts of Chemical Research 55 (21), 3125–3135 (2022)
A. Gautier

Isolating and engineering fluorescence-activating proteins using yeast surface display
Methods in Molecular Biology Vol. 2491, Yeast Surface Display (Traxlmayr, M., ed), pp 593-626, Humana, New York, NY (2022)  
L. El Hajji, H. Benaissa & A. Gautier

An expanded palette of fluorogenic HaloTag probes with enhanced contrast for targeted cellular imaging
Organic & Biomolecular Chemistry 20, 3619–3628 (2022)
S.P.J.T. Bachollet, Y. Shpinov, F. Broch, H. Benaissa, A. Gautier, N. Pietrancosta, J.-M. Mallet & B. Dumat

Reciprocal regulation of Shh Trafficking and H2Olevels via a noncanonical BOC-Rac1 pathway
Antioxidants 11, 718 (2022)
M. Thauvin, I. Amblard, C. Rampon, A. Mourton, I. Queguiner, C. Li, A. Gautier, A. Joliot, M. Volovitch, S. Vriz


Engineering of a fluorescent chemogenetic reporter with tunable color for advanced live-cell imaging.
Nature Communications 12, 6989 (2021)
H. Benaissa, K. Ounoughi, I. Aujard, E. Fischer, R. Goïame, J. Nguyen, A. G. Tebo, C. Li, T. Le Saux, L. Danglot, N. Pietrancosta, X. Morin, L. Jullien & A Gautier

Versatile on-demand fluorescent labeling of fusion proteins using Fluorescence-Activating and absorption-Shifting Tag (FAST).
Methods in Molecular Biology Vol. 2350, Multiplexed Imaging: Methods and Protocols (Zamir, E., ed), pp 253-265, Humana, New York, NY (2021).
A. Gautier, L. Jullien, C. Li, M.-A. Plamont, A. G. Tebo, M. Thauvin, M. Volovitch & S. Vriz

Orthogonal fluorescent chemogenetic reporters for multicolor imaging
Nature Chemical Biology 17, 30-38 (2021)
A. G. Tebo, B. Moeyaert, M. Thauvin, I. Carlon-Andres, D. Böken, M. Volovitch, S. Padilla-Parra, P. Dedecker, S. Vriz & A. Gautier


An alternative replication niche for Listeria monocytogenes revealed by fluorescent tagging of bacterial secreted effectors
PLoS Pathogens 16 (10), e1009001 (2020)
C. Peron Cane, J. Leblanc, L. Wingertsmann, J. Carlos Fernandez, A. Gautier, N. Desprat & A. Lebreton

Visualizing the dynamics of exported bacterial proteins with the chemogenetic fluorescent reporter FAST
Scientific Reports 10, 15791 (2020)
Y. Chekli, C. Peron-Cane, D. Dell’Arciprete, J.-F. Allemand, C. Li, J.-M. Ghigo, A. Gautier, A. Lebreton, N. Desprat & C. Beloin

A far-red fluorescent chemogenetic reporter for in vivo molecular imaging
Angewandte Chemie International Edition 59, 17917–17923 (2020)
C. Li, A. G. Tebo, M. Thauvin, M.-A. Plamont, M. Volovitch, X. Morin, S. Vriz & A. Gautier

Engineering glowing chemogenetic hybrids for spying on cells
European Journal of Organic Chemistry 35, 5637–5646 (2020)
H. Ben Aissa & A. Gautier

Illuminating cellular biochemistry: fluorogenic chemogenetic biosensors for biological imaging
ChemPlusChem 85, 1487–1497 (2020)
F. Broch & A. Gautier

Sensing cellular biochemistry with fluorescent chemical-genetic hybrids
Current Opinion in Chemical Biology 57, 58–64 (2020)
A. Gautier & A. G. Tebo


Next-generation fluorogen-based reporters and biosensors for advanced bioimaging
International Journal of Molecular Sciences 20, 6142 (2019)
T. Péresse & A. Gautier

A split fluorescent reporter with rapid and reversible complementation
Nature Communications 10, 2822 (2019) 
A. G. Tebo & A. Gautier

Single-molecule localization microscopy with the Fluorescence-Activating and absorption-Shifting Tag (FAST) system
ACS Chemical Biology 14 (6), 1115-1120 (2019)
E. M. Smith, A. Gautier & E. M. Puchner

Simple imaging protocol for autofluorescence elimination and optical sectioning in fluorescence endomicroscopy
Optica 6, 972-980 (2019).
R. Zhang, R. Chouket, A. G. Tebo, M.-A. Plamont, Z. Kelemen, L. Gissot, J.-D. Faure, A. Gautier, V. Croquette, L. Jullien & T. Le Saux

2nd PSL Chemical Biology Symposium (2019): at the crossroads of Chemistry and Biology
ChemBioChem 20, 968-973 (2019)
M. Lucchino, A. Billet, A. Versini, H. Bavireddi, B.-D. Dasari, S. Debieu, L. Colombeau, T. Canneque, A. Wagner, G. Masson, F. Taran, P. Karoyan, M. Delpierre, C. Gaillet, A. Houdusse, S. Britton, F. Schmidt, J.-C. Florent, P. Belmont, D. Monchaud, J. Cossy, C. Thomas, A. Gautier, L. Johannes, R. Rodriguez

Live cell super-resolution imaging by radial fluctuations using fluorogen binding tags
Nanoscale 11, 3626-3632 (2019)  
M. Venkatachalapathy, V. Belapurkar, M. Jose, A. Gautier & D. Nair

The glowing panoply of fluorogen-based markers for advanced bioimaging.
In Optogenetics: light-driven actuators and light-emitting sensors in cell biology, Comprehensive Series in Photochemical and Photobiological Sciences (S. Vriz & T. Ozawa Eds), pp 41-62, The Royal Society of Chemistry (2019).
A. Gautier


Spying on cells with chemical-genetic hybrids (Espionner les cellules avec des hybrides chémogénétiques)
L’Actualité Chimique 435, 31–35 (2018)
A. Gautier

Macroscale fluorescence imaging against autofluorescence under ambient light
Light: Science & Applications 7, 97 (2018)
R. Zhang, R. Chouket, M.-A. Plamont, Z. Kelemen, A. Espagne, A. G. Tebo, A. Gautier, L. Gissot, J.-D. Faure, L. Jullien, V. Croquette & T. Le Saux

Improved chemical-genetic fluorescent markers for protein imaging in living cells.
Biochemistry 57, 5648–5653 (2018)
A. G. Tebo, F. M. Pimenta, Y. Zhang & A. Gautier

Fluorogenic protein-based strategies for detection, actuation and sensing.
BioEssays 40,1800118 (2018)
A. Gautier & A. G. Tebo

Circularly permuted fluorogenic proteins for the design of modular biosensors.
ACS Chemical Biology 13, 2392–2397 (2018)
A. G. Tebo, F. M. Pimenta, M. Zoumpoulaki, C. Kikuti, H. Sirkia, M.-A. Plamont, A. Houdusse & A. Gautier

The inducible chemical-genetic fluorescent marker FAST outperforms classical fluorescent proteins for the accurate quantitative monitoring of bacterial biofilm dynamics.
Scientific Reports 8, 10336 (2018) 
A. Monmeyran, P. Thomen, H. Jonquière, F. Sureau, C. Douarche, C. Li, M.-A. Plamont, J.-F. Casella, A. Gautier & N. Henry

Fluorogenic probing of membrane protein trafficking.
Bioconjugate Chemistry 29, 1823–1828 (2018)
C. Li, A. Mourton, M.-A. Plamont, V. Rodrigues, I. Aujard, M. Volovitch, T. Le Saux, F. Perez, S. Vriz, L. Jullien, A. Joliot & A. Gautier


Resonant out-of-phase fluorescence microscopy and remote imaging overcome spectral limitations.
Nature Communications 8, 969 (2017)
J. Querard, R. Zhang, Z. Kelemen, M.-A. Plamont, X. Xie, R. Chouket, I. Roemgens, Y. Koperina, S. Albright, E. Ipendey, M. Volovitch, H. L. Sladitschek, P. Neveu, L. Gissot, A. Gautier, J.-D. Faure, V. Croquette, T. Le Saux & L. Jullien

Chromophore renewal and fluorogen-binding tags: a match made to last.
Scientific Reports 7, 12316 (2017)
F. M. Pimenta, G. Chiappetta, T. Le Saux, J. Vihn, L. Jullien & A. Gautier

Fluorogenic labeling strategies for biological imaging.
International Journal of Molecular Sciences 18, 1473 (2017)
C. Li, A. G. Tebo & A. Gautier

Hybrid fluorescent probes for imaging cellular proteins on demand.
Médecine/Sciences 33 (6-7), 576-578 (2017)
L. Jullien & A. Gautier

Dynamic multi-color protein labeling in living cells.
Chemical Science 8, 5598–5605 (2017) 
C. Li, M.-A. Plamont, H. Sladitschek, V. Rodrigues, I. Aujard, P. Neveu, T. Le Saux, L. Jullien & A. Gautier

PSL Chemical Biology Symposium First 2016 Edition: When Chemistry and Biology share the language of discovery.
ChemBioChem 18, 883-887 (2017)
A. Gautier & R. Rodriguez


Design and characterization of red fluorogenic push-pull chromophores holding great potential for biosensing and imaging.
Organic and Biomolecular Chemistry 14, 9253-9261 (2016)
C. Li, M.-A. Plamont, I. Aujard, T. Le Saux, L. Jullien & A. Gautier

Kinetics of reactive modules adds discriminative dimensions for selective cell imaging.
ChemPhysChem 17, 1396-1413 (2016)
J. Quérard, T. Le Saux, A. Gautier, D. Alcor, V. Croquette, C. Gosse, A. Lemarchand & L. Jullien

A small fluorescence-activating and absorption-shifting tag for tunable protein imaging in vivo.
Proceedings of the National Academy of Sciences (PNAS) 113 (3), 497-502 (2016)   
M.-A. Plamont, E. Billon-Denis, S. Maurin, C. Gauron, F. M. Pimenta, C. G. Specht, J. Shi, J. Querard, B. Pan, J. Rossignol, K. Moncoq, N. Morellet, M. Volovitch, E. Lescop, Y. Chen, A. Triller, S. Vriz, T. Le Saux, L. Jullien & A. Gautier
Highlighted in ‘This week in PNAS’ in PNAS 113 (3), 465-467 (2016).


Fluorogen-based reporters for fluorescence imaging: a review.
Methods and Applications in Fluorescence 3, 042007 (2015)
L. Jullien & A. Gautier

Light-activated proteolysis for the spatiotemporal control of proteins.
ACS Chemical Biology 10, 1643-1647 (2015) 
Q. Delacour, C. Li, M.-A. Plamont, E. Billon-Denis, I. Aujard, T. Le Saux, L. Jullien & A. Gautier

Expanding discriminative dimensions for analysis and imaging.
Chemical Science 6, 2968-2978 (2015) 
J. Querard, A. Gautier*, T. Le Saux* & L. Jullien*

Photoswitching kinetics and phase sensitive detection add discriminative dimensions for selective fluorescence imaging.
Angewandte Chemie International Edition 54, 2633-2637 (2015)
J. Querard, T.-Z. Markus, M.-A. Plamont, C. Gauron, P. Wang, A. Espagne, M. Volovitch, S. Vriz, V. Croquette, A. Gautier*, T. Le Saux* & L. Jullien*

Preface. Site-specific protein labeling
Site-Specific Protein Labeling: Methods and Protocols, Methods in Molecular Biology, Vol. 1266 (Gautier, A. & Hinner M. J., eds), pp v-viii, Humana Press, USA (2015)
A. Gautier & M. J. Hinner

Site-specific protein labeling, Methods and Protocols series (Book)
Methods in Molecular Biology, Vol. 1266, Humana Press, USA (2015)
A. Gautier & M. J. Hinner (Editors)


How to control proteins with light in living systems.
Nature Chemical Biology 10, 533-541 (2014) 
A. Gautier, C. Gauron, M. Volovitch, D. Bensimon, L. Jullien & S. Vriz

Nitric oxide-triggered remodeling of chloroplast bioenergetics and thylakoid proteins upon nitrogen starvation in Chlamydomonas reinhardtii.
Plant Cell 26, 353-372 (2014)
L. Wei, B. Derrien, A. Gautier, L. Houille-Vernes, A. Boulouis, D. Saint-Marcoux, A. Malnoe, F. Rappaport, C. de Vitry, O. Vallon, Y. Choquet & F.-A. Wollman


Photochemical properties of Spinach and its use in selective imaging.
Chemical Science 4, 2865-2873 (2013) 
P. Wang, J. Querard, S. Maurin, S. S. Nath, T. Le Saux*, A. Gautier* & L. Jullien*


Self-immolation for uncaging with fluorescence reporting.
Angewandte Chemie International Edition 57, 9344-9347 (2012) 
R. Labruère, A. Alouane, T. Le Saux, I. Aujard, P. Pelupessy, A. Gautier, S. Dubruille, F. Schmidt & L. Jullien

Modification-free photocontrol of β-lactam conversion with spatio-temporal resolution.
ACS Synthetic Biology 1, 526-531 (2012) 
A. Venancio-Marques, Y.-J. Liu, A. Diguet, T. di Maio, A. Gautier & D. Baigl


Light-activated kinases enable the temporal dissection of signaling networks in living cells.
 Journal of the American Chemical Society 133, 2124-2127 (2011) 
A. Gautier, A. Deiters & J.W. Chin


Genetically encoded photocontrol of protein localization in mammalian cells.
Journal of the American Chemical Society 132, 4086-4088 (2010) 
A. Gautier, D.P. Nguyen, H. Lusic, W. An, A. Deiters & J.W. Chin


Selective crosslinking of interacting proteins using self-labeling tags.
Journal of the American Chemical Society 131, 17954-17962 (2009) 
A. Gautier, E. Nakata, G. Lukinavičius, K.T. Tan & K. Johnsson

The N-C=O interaction: a weak bond with attractive properties for the deliberate exploitation in medicinal and supramolecular chemistry.
In Targets in heterocyclic systems – Chemistry and Properties, Volume 13 (Attanasi, O.A., Spinelli, D., eds.), pp. 1-26, The Royal Society of Chemistry (2009)
J. Hasserodt, A. Gautier, R. Barbe & M. Waibel


AGT/SNAP-tag: a versatile tag for covalent protein labeling.
In Probes and tags to study biomolecular function: for proteins, RNA and membranes (Miller, L.W., ed.), pp. 89-107, Wiley-VCH, Weinheim (2008)
A. Gautier, K. Johnsson & H.M. O’Hare

An engineered protein tag for multiprotein labeling in living cells.
Chemistry & Biology 15, 128-136 (2008) 
A. Gautier, A. Juillerat, C. Heinis, I.R. Correa, M. Kindermann, F. Beaufils & K. Johnsson


Chemical probes shed light on protein function.
Current Opinion in Structural Biology 17, 488-494 (2007) 
H. M. O’Hare, K. Johnsson & A. Gautier

Observing and manipulating proteins in living cells – Covalent labeling of fusion proteins.
BIOforum Europe 11, 38-40 (2007)
K. Johnsson, H. O’Hare & A. Gautier


An unusual functional group interaction and its potential to reproduce steric and electrostatic features of the transition states of peptidolysis.
Bioorganic & Medicinal Chemistry 14, 3835-3847 (2006)
A. Gautier, D. Pitrat & J. Hasserodt


Chiral trialkanolamine-based hemicryptophanes: Synthesis and oxovanadium complex.
Organic Letters 7, 1207-1210 (2005) 
A. Gautier, J.-C. Mulatier, J. Crassous & J.-P Dutasta