The overall goal of my research is to develop molecules and methods to study biological processes in all their complexity. My research focuses on the development of chemical tools to observe and manipulate biological processes in new ways, specifically pairing tailor-made synthetic molecules with engineered genetically encodable biomolecules with the goal of obtaining selective and controlled output functions. After postdoctoral research dealing with new approaches to site-specifically modify proteins with chemical functionalities in live cells, I focused my early independent research on the development of new tools to advance biomolecular imaging and chemical optogenetics. My current and future research aims at pushing back the frontiers of biological imaging in order to interrogate quantitatively and comprehensively living systems at the molecular, cellular and network levels. This includes the design of innovative probes and methods to advance multiplexing and increase the spatiotemporal resolution of live-cell imaging.