PREPRINT Early HIV-1 maturation drives Env clustering and fusion competence
bioRxiv https://doi.org/10.64898/2025.12.11.693442
I. Carlon-Andres, V. Garcia-Giner, D. J. Villiamson, R. Teja Ravi, A. Le Bas, P. N. Ward, T. Starling, L. El Hajji, A. Gautier, S. Simoncelli, M. Grange, M. Dumoux & S. Padilla-Parra
PREPRINT Photoejection turns non-covalent fluorescent tags into negative reversible photoswitchers
bioRxiv https://doi.org/10.64898/2025.12.05.692574
Y. Shpinov, M. Mandal, V. van Deuren, A. Lahlou, M. Le Bec, R. Chouket, C. Hadj Moussa, C. Bonin, H. Sepasi Tehrani, I. Coghill, L. El Hajji, K. Ounoughi, J. Franco Pinto, M.-A. Plamont, P. Pelupessy, I. Ayala, F. Perez, I. Aujard, T. Le Saux, A. Gautier, P. Dedecker, B. Brutscher, Ludovic Jullien
PREPRINT Characterizing drug activity with sensitive interactomes in human living cells
bioRxiv https://doi.org/10.1101/2025.11.20.689448
C. Kundlacz, N. Hajj Sleiman, F. Marmigère, B. Gillet, S. Hughes, L. Gilquin, H. Duplus, G. Yvert, F. Bleicher, A. Gautier, I. Coste, T. Renno & S. Merabet
PREPRINT Chemogenetic modulation of luciferase emission color for imaging and sensing
bioRxiv https://doi.org/10.1101/2025.11.21.689291
H. Manirakiza, Y. Shpinov, A. Gontier, L. El Hajji, L. Jullien & A. Gautier
PREPRINT Chemically responsive protein switches for the precise control of biological activities
bioRxiv https://doi.org/10.1101/2025.05.16.654305
J. Franco Pinto, N. Drahy, Séverine Divoux, F. Perez & A. Gautier
PREPRINT Peptide-functionalized fluorescent polymeric nanoparticles: polysarcosine length defines stealth properties
bioRxiv https://doi.org/10.1101/2025.04.20.649688
T. Gupta, B. Quynh Anh, H. Manirakiza, L. El Hajji, N. Humbert, A. Wahab Mouhamad, A. Reisch, A. Gautier, A. S. Klymchenko
2025
Unequal mitochondrial segregation promotes asymmetric fates during neurogenesis
Nature Communications 16, 11049 (2025)
B. Bunel, R. Leclercq, R. Goiame, A. Gautier, X. Morin, E. Fischer
Super-resolution live-cell mapping of protein-protein interactions using chemogenetic split reporters and STED microscopy
ChemBioChem e202500754 (2025)
S. Board & A. Gautier
Design and evaluation of a tripartite chemogenetic fluorescent reporter for visualizing ternary protein complexes
Nature Communications 16, 6955 (2025)
S. Bottone, F. Broch, A. Gedeon, A. Brion, L. El Hajji, H. Benaissa & A. Gautier
Engineering, design and selection of fluorescence-activating proteins for advanced imaging and biosensing
Protein Engineering, Design and Selection 38, gzaf007 (2025)
L. El Hajji & A. Gautier
A tunable and versatile near infrared fluorescent chemogenetic reporter
Nature Communications 16, 2594 (2025)
L. El Hajji, B. Bunel, O. Joliot, C. Li, A. G. Tebo, C. Rampon, M. Volovitch, E. Fischer, N. Pietrancosta, F. Perez, X. Morin, S. Vriz & A. Gautier
2024
Simultaneous detection of membrane contact dynamics and associated Ca2+ signals by reversible chemogenetic reporters
Nature Communications 15, 9775 (2024)
P. Garcia Casas, M. Rossini, L. Pavenius, M. Saeed, N. Arnst, S. Sonda, M. Bruzzone, V. Berno, A. Raimoindi, M. Livia Sassano, L. Naia, P. Agostinis, M. Sturlese, B. Niemeyer, H. Brisman, M. Ankarcrona, A. Gautier, P. Pizzo, R. Filadi
Multiplexed in vivo imaging using fluorescence lifetime modulating tags
Advanced Science 2404354 (2024)
L. El Hajji, F. Lam, M. Avtodeeva, H. Benaissa, C. Rampon, M. Volovitch, S. Vriz & A. Gautier
Improving split reporters of protein-protein interactions through orthology-based protein engineering
ACS Chemical Biology 19, 428–441 (2024)
L.-M. Rakotoarison, A. G. Tebo, D. Böken, S. Board, L. El Hajji & A. Gautier
2023
Engineering of tunable allosteric fluorogenic protein biosensors
ACS Sensors 8, 3933–3942 (2023)
F. Broch, L. El Hajji, N. Pietrancosta & A. Gautier
A fluorogenic chemically induced dimerization technology for controlling, imaging and sensing protein proximity
Nature Methods 20, 1553-1562(2023)
S. Bottone, O. Joliot, Z.V. Cakil, L. El Hajji, L.-M. Rakotoarison, G. Boncompain, F. Perez & A. Gautier
A fluorescence reporter system for anaerobic thermophiles
Frontiers in Bioengineering and Biotechnology 11, 1226889 (2023)
R. Hocq, S. Bottone, A. Gautier, S. Pflügl
Genetic targeting of solvatochromic dyes for probing nanoscale environment of proteins in organelles
Analytical Chemistry 95 (22), 8512–8521 (2023)
R. Pelletier; D. I. Danylchuk, H. Benaissa, F. Broch, R. Vauchelles, A. Gautier, A. S. Klymchenko
2022
Fluorescence-activating and absorption-shifting tags for advanced imaging and biosensing
Accounts of Chemical Research 55 (21), 3125–3135 (2022)
A. Gautier
Isolating and engineering fluorescence-activating proteins using yeast surface display
Methods in Molecular Biology Vol. 2491, Yeast Surface Display (Traxlmayr, M., ed), pp 593-626, Humana, New York, NY (2022)
L. El Hajji, H. Benaissa & A. Gautier
An expanded palette of fluorogenic HaloTag probes with enhanced contrast for targeted cellular imaging
Organic & Biomolecular Chemistry 20, 3619–3628 (2022)
S.P.J.T. Bachollet, Y. Shpinov, F. Broch, H. Benaissa, A. Gautier, N. Pietrancosta, J.-M. Mallet & B. Dumat
Reciprocal regulation of Shh Trafficking and H2O2 levels via a noncanonical BOC-Rac1 pathway
Antioxidants 11, 718 (2022)
M. Thauvin, I. Amblard, C. Rampon, A. Mourton, I. Queguiner, C. Li, A. Gautier, A. Joliot, M. Volovitch, S. Vriz
2021
Engineering of a fluorescent chemogenetic reporter with tunable color for advanced live-cell imaging.
Nature Communications 12, 6989 (2021)
H. Benaissa, K. Ounoughi, I. Aujard, E. Fischer, R. Goïame, J. Nguyen, A. G. Tebo, C. Li, T. Le Saux, L. Danglot, N. Pietrancosta, X. Morin, L. Jullien & A Gautier
Versatile on-demand fluorescent labeling of fusion proteins using Fluorescence-Activating and absorption-Shifting Tag (FAST).
Methods in Molecular Biology Vol. 2350, Multiplexed Imaging: Methods and Protocols (Zamir, E., ed), pp 253-265, Humana, New York, NY (2021).
A. Gautier, L. Jullien, C. Li, M.-A. Plamont, A. G. Tebo, M. Thauvin, M. Volovitch & S. Vriz
Orthogonal fluorescent chemogenetic reporters for multicolor imaging
Nature Chemical Biology 17, 30-38 (2021)
A. G. Tebo, B. Moeyaert, M. Thauvin, I. Carlon-Andres, D. Böken, M. Volovitch, S. Padilla-Parra, P. Dedecker, S. Vriz & A. Gautier
2020
An alternative replication niche for Listeria monocytogenes revealed by fluorescent tagging of bacterial secreted effectors
PLoS Pathogens 16 (10), e1009001 (2020)
C. Peron Cane, J. Leblanc, L. Wingertsmann, J. Carlos Fernandez, A. Gautier, N. Desprat & A. Lebreton
Visualizing the dynamics of exported bacterial proteins with the chemogenetic fluorescent reporter FAST
Scientific Reports 10, 15791 (2020)
Y. Chekli, C. Peron-Cane, D. Dell’Arciprete, J.-F. Allemand, C. Li, J.-M. Ghigo, A. Gautier, A. Lebreton, N. Desprat & C. Beloin
A far-red fluorescent chemogenetic reporter for in vivo molecular imaging
Angewandte Chemie International Edition 59, 17917–17923 (2020)
C. Li, A. G. Tebo, M. Thauvin, M.-A. Plamont, M. Volovitch, X. Morin, S. Vriz & A. Gautier
Engineering glowing chemogenetic hybrids for spying on cells
European Journal of Organic Chemistry 35, 5637–5646 (2020)
H. Ben Aissa & A. Gautier
Illuminating cellular biochemistry: fluorogenic chemogenetic biosensors for biological imaging
ChemPlusChem 85, 1487–1497 (2020)
F. Broch & A. Gautier
Sensing cellular biochemistry with fluorescent chemical-genetic hybrids
Current Opinion in Chemical Biology 57, 58–64 (2020)
A. Gautier & A. G. Tebo
2019
Next-generation fluorogen-based reporters and biosensors for advanced bioimaging
International Journal of Molecular Sciences 20, 6142 (2019)
T. Péresse & A. Gautier
A split fluorescent reporter with rapid and reversible complementation
Nature Communications 10, 2822 (2019)
A. G. Tebo & A. Gautier
Single-molecule localization microscopy with the Fluorescence-Activating and absorption-Shifting Tag (FAST) system
ACS Chemical Biology 14 (6), 1115-1120 (2019)
E. M. Smith, A. Gautier & E. M. Puchner
Simple imaging protocol for autofluorescence elimination and optical sectioning in fluorescence endomicroscopy
Optica 6, 972-980 (2019).
R. Zhang, R. Chouket, A. G. Tebo, M.-A. Plamont, Z. Kelemen, L. Gissot, J.-D. Faure, A. Gautier, V. Croquette, L. Jullien & T. Le Saux
2nd PSL Chemical Biology Symposium (2019): at the crossroads of Chemistry and Biology
ChemBioChem 20, 968-973 (2019)
M. Lucchino, A. Billet, A. Versini, H. Bavireddi, B.-D. Dasari, S. Debieu, L. Colombeau, T. Canneque, A. Wagner, G. Masson, F. Taran, P. Karoyan, M. Delpierre, C. Gaillet, A. Houdusse, S. Britton, F. Schmidt, J.-C. Florent, P. Belmont, D. Monchaud, J. Cossy, C. Thomas, A. Gautier, L. Johannes, R. Rodriguez
Live cell super-resolution imaging by radial fluctuations using fluorogen binding tags
Nanoscale 11, 3626-3632 (2019)
M. Venkatachalapathy, V. Belapurkar, M. Jose, A. Gautier & D. Nair
The glowing panoply of fluorogen-based markers for advanced bioimaging.
In Optogenetics: light-driven actuators and light-emitting sensors in cell biology, Comprehensive Series in Photochemical and Photobiological Sciences (S. Vriz & T. Ozawa Eds), pp 41-62, The Royal Society of Chemistry (2019).
A. Gautier
2018
Spying on cells with chemical-genetic hybrids (Espionner les cellules avec des hybrides chémogénétiques)
L’Actualité Chimique 435, 31–35 (2018)
A. Gautier
Macroscale fluorescence imaging against autofluorescence under ambient light
Light: Science & Applications 7, 97 (2018)
R. Zhang, R. Chouket, M.-A. Plamont, Z. Kelemen, A. Espagne, A. G. Tebo, A. Gautier, L. Gissot, J.-D. Faure, L. Jullien, V. Croquette & T. Le Saux
Improved chemical-genetic fluorescent markers for protein imaging in living cells.
Biochemistry 57, 5648–5653 (2018)
A. G. Tebo, F. M. Pimenta, Y. Zhang & A. Gautier
Fluorogenic protein-based strategies for detection, actuation and sensing.
BioEssays 40,1800118 (2018)
A. Gautier & A. G. Tebo
Circularly permuted fluorogenic proteins for the design of modular biosensors.
ACS Chemical Biology 13, 2392–2397 (2018)
A. G. Tebo, F. M. Pimenta, M. Zoumpoulaki, C. Kikuti, H. Sirkia, M.-A. Plamont, A. Houdusse & A. Gautier
The inducible chemical-genetic fluorescent marker FAST outperforms classical fluorescent proteins for the accurate quantitative monitoring of bacterial biofilm dynamics.
Scientific Reports 8, 10336 (2018)
A. Monmeyran, P. Thomen, H. Jonquière, F. Sureau, C. Douarche, C. Li, M.-A. Plamont, J.-F. Casella, A. Gautier & N. Henry
Fluorogenic probing of membrane protein trafficking.
Bioconjugate Chemistry 29, 1823–1828 (2018)
C. Li, A. Mourton, M.-A. Plamont, V. Rodrigues, I. Aujard, M. Volovitch, T. Le Saux, F. Perez, S. Vriz, L. Jullien, A. Joliot & A. Gautier
2017
Resonant out-of-phase fluorescence microscopy and remote imaging overcome spectral limitations.
Nature Communications 8, 969 (2017)
J. Querard, R. Zhang, Z. Kelemen, M.-A. Plamont, X. Xie, R. Chouket, I. Roemgens, Y. Koperina, S. Albright, E. Ipendey, M. Volovitch, H. L. Sladitschek, P. Neveu, L. Gissot, A. Gautier, J.-D. Faure, V. Croquette, T. Le Saux & L. Jullien
Chromophore renewal and fluorogen-binding tags: a match made to last.
Scientific Reports 7, 12316 (2017)
F. M. Pimenta, G. Chiappetta, T. Le Saux, J. Vihn, L. Jullien & A. Gautier
Fluorogenic labeling strategies for biological imaging.
International Journal of Molecular Sciences 18, 1473 (2017)
C. Li, A. G. Tebo & A. Gautier
Hybrid fluorescent probes for imaging cellular proteins on demand.
Médecine/Sciences 33 (6-7), 576-578 (2017)
L. Jullien & A. Gautier
Dynamic multi-color protein labeling in living cells.
Chemical Science 8, 5598–5605 (2017)
C. Li, M.-A. Plamont, H. Sladitschek, V. Rodrigues, I. Aujard, P. Neveu, T. Le Saux, L. Jullien & A. Gautier
PSL Chemical Biology Symposium First 2016 Edition: When Chemistry and Biology share the language of discovery.
ChemBioChem 18, 883-887 (2017)
A. Gautier & R. Rodriguez
2016
Design and characterization of red fluorogenic push-pull chromophores holding great potential for biosensing and imaging.
Organic and Biomolecular Chemistry 14, 9253-9261 (2016)
C. Li, M.-A. Plamont, I. Aujard, T. Le Saux, L. Jullien & A. Gautier
Kinetics of reactive modules adds discriminative dimensions for selective cell imaging.
ChemPhysChem 17, 1396-1413 (2016)
J. Quérard, T. Le Saux, A. Gautier, D. Alcor, V. Croquette, C. Gosse, A. Lemarchand & L. Jullien
A small fluorescence-activating and absorption-shifting tag for tunable protein imaging in vivo.
Proceedings of the National Academy of Sciences (PNAS) 113 (3), 497-502 (2016)
M.-A. Plamont, E. Billon-Denis, S. Maurin, C. Gauron, F. M. Pimenta, C. G. Specht, J. Shi, J. Querard, B. Pan, J. Rossignol, K. Moncoq, N. Morellet, M. Volovitch, E. Lescop, Y. Chen, A. Triller, S. Vriz, T. Le Saux, L. Jullien & A. Gautier
Highlighted in ‘This week in PNAS’ in PNAS 113 (3), 465-467 (2016).
2015
Fluorogen-based reporters for fluorescence imaging: a review.
Methods and Applications in Fluorescence 3, 042007 (2015)
L. Jullien & A. Gautier
Light-activated proteolysis for the spatiotemporal control of proteins.
ACS Chemical Biology 10, 1643-1647 (2015)
Q. Delacour, C. Li, M.-A. Plamont, E. Billon-Denis, I. Aujard, T. Le Saux, L. Jullien & A. Gautier
Expanding discriminative dimensions for analysis and imaging.
Chemical Science 6, 2968-2978 (2015)
J. Querard, A. Gautier*, T. Le Saux* & L. Jullien*
Photoswitching kinetics and phase sensitive detection add discriminative dimensions for selective fluorescence imaging.
Angewandte Chemie International Edition 54, 2633-2637 (2015)
J. Querard, T.-Z. Markus, M.-A. Plamont, C. Gauron, P. Wang, A. Espagne, M. Volovitch, S. Vriz, V. Croquette, A. Gautier*, T. Le Saux* & L. Jullien*
Preface. Site-specific protein labeling
Site-Specific Protein Labeling: Methods and Protocols, Methods in Molecular Biology, Vol. 1266 (Gautier, A. & Hinner M. J., eds), pp v-viii, Humana Press, USA (2015)
A. Gautier & M. J. Hinner
Site-specific protein labeling, Methods and Protocols series (Book)
Methods in Molecular Biology, Vol. 1266, Humana Press, USA (2015)
A. Gautier & M. J. Hinner (Editors)
2014
How to control proteins with light in living systems.
Nature Chemical Biology 10, 533-541 (2014)
A. Gautier, C. Gauron, M. Volovitch, D. Bensimon, L. Jullien & S. Vriz
Nitric oxide-triggered remodeling of chloroplast bioenergetics and thylakoid proteins upon nitrogen starvation in Chlamydomonas reinhardtii.
Plant Cell 26, 353-372 (2014)
L. Wei, B. Derrien, A. Gautier, L. Houille-Vernes, A. Boulouis, D. Saint-Marcoux, A. Malnoe, F. Rappaport, C. de Vitry, O. Vallon, Y. Choquet & F.-A. Wollman
2013
Photochemical properties of Spinach and its use in selective imaging.
Chemical Science 4, 2865-2873 (2013)
P. Wang, J. Querard, S. Maurin, S. S. Nath, T. Le Saux*, A. Gautier* & L. Jullien*
2012
Self-immolation for uncaging with fluorescence reporting.
Angewandte Chemie International Edition 57, 9344-9347 (2012)
R. Labruère, A. Alouane, T. Le Saux, I. Aujard, P. Pelupessy, A. Gautier, S. Dubruille, F. Schmidt & L. Jullien
Modification-free photocontrol of β-lactam conversion with spatio-temporal resolution.
ACS Synthetic Biology 1, 526-531 (2012)
A. Venancio-Marques, Y.-J. Liu, A. Diguet, T. di Maio, A. Gautier & D. Baigl
2011
Light-activated kinases enable the temporal dissection of signaling networks in living cells.
Journal of the American Chemical Society 133, 2124-2127 (2011)
A. Gautier, A. Deiters & J.W. Chin
2010
Genetically encoded photocontrol of protein localization in mammalian cells.
Journal of the American Chemical Society 132, 4086-4088 (2010)
A. Gautier, D.P. Nguyen, H. Lusic, W. An, A. Deiters & J.W. Chin
2009
Selective crosslinking of interacting proteins using self-labeling tags.
Journal of the American Chemical Society 131, 17954-17962 (2009)
A. Gautier, E. Nakata, G. Lukinavičius, K.T. Tan & K. Johnsson
The N-C=O interaction: a weak bond with attractive properties for the deliberate exploitation in medicinal and supramolecular chemistry.
In Targets in heterocyclic systems – Chemistry and Properties, Volume 13 (Attanasi, O.A., Spinelli, D., eds.), pp. 1-26, The Royal Society of Chemistry (2009)
J. Hasserodt, A. Gautier, R. Barbe & M. Waibel
2008
AGT/SNAP-tag: a versatile tag for covalent protein labeling.
In Probes and tags to study biomolecular function: for proteins, RNA and membranes (Miller, L.W., ed.), pp. 89-107, Wiley-VCH, Weinheim (2008)
A. Gautier, K. Johnsson & H.M. O’Hare
An engineered protein tag for multiprotein labeling in living cells.
Chemistry & Biology 15, 128-136 (2008)
A. Gautier, A. Juillerat, C. Heinis, I.R. Correa, M. Kindermann, F. Beaufils & K. Johnsson
2007
Chemical probes shed light on protein function.
Current Opinion in Structural Biology 17, 488-494 (2007)
H. M. O’Hare, K. Johnsson & A. Gautier
Observing and manipulating proteins in living cells – Covalent labeling of fusion proteins.
BIOforum Europe 11, 38-40 (2007)
K. Johnsson, H. O’Hare & A. Gautier
2006
An unusual functional group interaction and its potential to reproduce steric and electrostatic features of the transition states of peptidolysis.
Bioorganic & Medicinal Chemistry 14, 3835-3847 (2006)
A. Gautier, D. Pitrat & J. Hasserodt
2005
Chiral trialkanolamine-based hemicryptophanes: Synthesis and oxovanadium complex.
Organic Letters 7, 1207-1210 (2005)
A. Gautier, J.-C. Mulatier, J. Crassous & J.-P Dutasta
Chemogenetics to image and control cells 